In the 16th century, the linguistic development of the Croatian language was relatively stable despite the turbulent socio-political changes caused by population migrations. Despite migrations, wars, and destructions, Croatian literacy and literature remained steadfast. The central figure of the 16th century was Marko Marulić (1450-1524), who is considered the father of Croatian literature. He authored numerous well-known works such as Molitva suprotiva Turkom, with the pinnacle of his creation being the biblical epic Judita written in six cantos in the Split dialect of Chakavian and composed in double-rhymed dodecasyllables. In this paper, lexemes from Judita by Marko Marulić listed in the extensive corpus of Dictionary of Marulić's Judita (Rječnik Marulićeve Judite) by Milan Moguš (2001) are compared with those in the contemporary Great Dictionary of the Croatian Standard Language (Veliki rječnik hrvatskoga standardnog jezika). The paper examines whether the lexemes from the time of Marulić's Judita are still used in contemporary Croatian language and whether there are any semantic shifts in relation to their meanings recorded in the 16th century. It also identifies the layer of Marulić's lexemes that no longer exist in the contemporary language but are confirmed in older Croatian dictionaries.