Raising knowledge of environmental conservation and the necessary behavioural changes is largely accomplished through ecological education. Sustainable development is an important concept in preserving natural resources for future generations. The educational system and the media have a crucial role in educating young people about ecological issues, as proper information and education about ecology can help develop environmentally conscious behaviours and reduce harmful impacts on nature. An empirical study conducted at the Department of Teacher Education, Faculty of Humanities and Social Sciences in Split, examined students' ecological awareness. Quantitative research was conducted, and the survey method was applied. The questionnaire consisted of six parts, each offering a five-point Likert scale. The goal was to understand students' awareness of ecological concepts, explore their attitudes towards nature conservation, their concerns about environmental issues, and whether they have developed pro-environmental habits. The majority of Split Teacher Education students identify as environmentally aware, comprehend fundamental ecological ideas, and have shown an elevated level of concern for a range of environmental situations. Female students were found to be more active in pro-environmental behaviours compared to male students, while the year of study did not significantly affect students' pro-environmental habits. However, difficulties in understanding or practicing environmentally friendly behaviours in a smaller part of students were shown. It was observed that students with higher levels of ecological awareness are more likely to demonstrate pro-environmental behaviour. The research results provide insight into the current status of ecological awareness and behaviour of the Department of Teacher Education students in Split, but also reveal the need for support and suggest areas for further educational efforts and interventions to improve ecological knowledge and engagement, with an emphasis on raising awareness of the importance of caring for nature.